The diagnostic value of cyclophilin A and VAP-1 in patients with suspected pulmonary embolism

Aynur Sahin; Olgun Asik; Ozgur Tatli; Yunus Karaca; Selim Demir; Ahmet Mentese; Suleyman Caner Karahan; Suleyman Turedi

doi:10.21601/ortadogutipdergisi.441377

ORTADOĞU TIP DERGİSİ - ORTADOGU MEDICAL JOURNAL

The diagnostic value of cyclophilin A and VAP-1 in patients with suspected pulmonary embolism

Pulmoner emboli şüphesi olan hastalarda siklofilin A ve VAP-1’in tanısal değeri

Aynur Sahin ¹ ^* , Olgun Asik ², Ozgur Tatli ¹, Yunus Karaca ¹, Selim Demir ³, Ahmet Mentese ⁴, Suleyman Caner Karahan ⁵, Suleyman Turedi ¹

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Ortadogu Tıp Derg, Volume 10, Issue 3, pp. 228-233

https://doi.org/10.21601/ortadogutipdergisi.441377

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Abstract

Aim: To investigate the diagnostic value of the biochemical markers cyclophilin A (CYPA) and vascular adhesion protein-1 (VAP-1), associated with endothelial dysfunction, platelet adhesion, and arterial thrombus in pulmonary embolism (PE), in whose pathophysiology platelet activation and aggregation resulting from endothelial damage are known to be involved.
Material and Method: Serum CYPA and VAP-1 levels of 165 patients presenting to the emergency department with suspected acute PE and with presence of PE investigated at spiral computerized tomography angiography were measured in this prospective clinical study. These patients were assigned into two groups, PE (+) and PE (-), based on the computerized tomography results, and their CYPA and VAP-1 levels were then compared.
Results: Comparison of the two groups’ measurements revealed a significant difference in terms of VAP-1 levels (p=0.0001), but none for CYPA (p=0.381).
Conclusion: Diagnostic value was determined for serum VAP-1 levels in acute PE, but we couldn’t detect any diagnostic value for CYPA in patients with acute PE.

Keywords: Pulmonary embolism, VAP-1, cyclophilin A, VAP-1

Öz

Amaç: Bu çalışmanın amacı endotel hasarı sonucu trombosit aktivasyonu ve kümeleşmesinin patofizyolojisinde rol oynadığı bilinen pulmoner emboli (PE) hastalığında; endotel disfonksiyonu, platelet adezyonu ve arterial trombüs ile ilişkelendirilen siklofilin A (CYPA) ve vasküler adezyon protein- 1 (VAP-1) adlı biyokimyasal belirteçlerin tanısal değerini araştırmaktır.
Gereç ve Yöntem: Prospektif olarak yapılan bu klinik çalışmada acil servise akut PE şüphesi ile başvurarak çalışmaya alınan ve spiral bilgisayarlı tomografi anjiografi ile PE varlığı değerlendirilen 165 hastanın serum CYPA ve VAP-1 düzeyi ölçüldü. Bilgisayarlı tomografi sonuçlarına göre, PE (+) ve PE (-) olarak 2 gruba ayrılan bu hastaların ölçülen CYPA ve VAP-1 düzeyleri birbirleriyle karşılaştırıldı.
Bulgular: Her iki grup ölçümleri birbiriyle karşılaştırıldığında VAP-1 düzeyi bakımından gruplar arası fark istatistiksel anlamlı bulunurken (p=0.0001), CYPA düzeyinin bakımından bu fark istatistiksel olarak anlamlı değildi. (p=0.381).
Sonuç: Akut PE’de serum VAP-1 düzeyinin tanısal olarak kullanılabileceği tespit edilmiştir. Serum CYPA düzeyinin ise akut PE’de tanısal değeri bulunmamaktadır.

Keywords: Pulmoner emboli, siklofilin A, VAP-1

References

Konstantinides S, Torbicki A, Agnelli G, et al. ESC/PCS New Guidelines. Kardiol Pol 2014; 72: 997-1053.
Bova C, Greco F, Misuraca G, et al. Diagnostic utility of echocardiography in patients with suspected pulmonary embolism. Am J Emerg Med 2003; 21: 180-3.
Seizer P, Fuchs C, Ungern-Sternberg SN, et al. Platelet-bound cyclophilin A in patients with stable coronary artery disease and acute myocardial infarction. Platelets 2016; 27: 155-8. doi:10.3109/09537104.2015.1051466.
Elvers M, Herrmann A, Seizer P, et al. Intracellular cyclophilin A is an important Ca(2+) regulator in platelets and critically involved in arterial thrombus formation. Blood 2012; 120: 1317-26. doi:10.1182/blood-2011-12-398438.
Tian-Tian Z, Jun-Feng Z, Heng G. Functions of cyclophilin A in atherosclerosis. Exp Clin Cardiol 2013; 18:e118-24.
Pannecoeck R, Serruys D, Benmeridja L, et al. Vascular adhesion protein-1: Role in human pathology and application as a biomarker. Crit Rev Clin Lab Sci 2015; 52: 284-300. doi:10.3109/10408363.2015.1050714.
Smith DJ, Salmi M, Bono P, Hellman J, Leu T, Jalkanen S. Cloning of vascular adhesion protein 1 reveals a novel multifunctional adhesion molecule. J Exp Med 1998; 188: 17-27.
Aalto K, Maksimow M, Juonala M, et al. Soluble vascular adhesion protein-1 correlates with cardiovascular risk factors and early atherosclerotic manifestations. Arterioscler Thromb Vasc Biol 2012; 32: 52332.doi:10.1161/ATVBAHA.111.238030.
Altug Cakmak H, Aslan S, Erturk M, et al. Assessment of the Relationship Between Serum Vascular Adhesion Protein-1 (VAP-1) and Severity of Calcific Aortic Valve Stenosis. J Heart Valve Dis 2015; 24: 699-706.
Chen DW, Zhao RM, Jin Y, et al. Plasma soluble vascular adhesion protein-1 concentration correlates with arterial stiffness: A cross-sectional study. Arch Gerontol Geriatr 2015; 61: 67-71. doi:10.1016/j.archger.2015.04.007.
Airas L, Lindsberg PJ, Karjalainen-Lindsberg ML, et al. Vascular adhesion protein-1 in human ischaemic stroke. Neuropathol Appl Neurobiol 2008; 34: 394-402. doi:10.1111/j.1365-2990.2007.00911.x.
Aalto K, Havulinna AS, Jalkanen S, Salomaa V, Salmi M. Soluble vascular adhesion protein-1 predicts incident major adverse cardiovascular events and improves reclassification in a finnish prospective cohort study. Circ Cardiovasc Genet 2014; 7: 529-35. doi:10.1161/CIRCGENETICS.113.000543.
Sun P, Sole M, Unzeta M. Involvement of SSAO/VAP-1 in oxygen-glucose deprivation-mediated damage using the endothelial hSSAO/VAP-1-expressing cells as experimental model of cerebral ischemia. Cerebrovasc Dis 2014; 37: 171-80. doi:10.1159/000357660.
Ma Q, Manaenko A, Khatibi NH, Vascular adhesion protein-1 inhibition provides antiinflammatory protection after an intracerebral hemorrhagic stroke in mice. J Cereb Blood Flow Metab 2011; 31: 881-93. doi:10.1038/jcbfm.2010.167.
Karaca Y, Tatli O, Aksut N, et al. Diagnostic Value of Cyclophilin A in Acute Ischemic Stroke. Eurasian J Emerg Med 2017; 16: 54-6. doi:10.5152/eajem.2017.35119.
Nigro P, Satoh K, O'Dell MR, et al. Cyclophilin A is an inflammatory mediator that promotes atherosclerosis in apolipoprotein E-deficient mice. J Exp Med 2011; 208: 53-66. doi:10.1084/jem.20101174.
Satoh K, Nigro P, Matoba T, O'Dell MR, Cui Z, Shi X et al. Cyclophilin A enhances vascular oxidative stress and the development of angiotensin II-induced aortic aneurysms. Nat Med. 2009; 15: 649-56. doi:10.1038/nm.1958.
Jin ZG, Melaragno MG, Liao DF, et al. Cyclophilin A is a secreted growth factor induced by oxidative stress. Circ Res 2000; 87: 789-96.
Liao DF, Jin ZG, Baas AS, et al. Purification and identification of secreted oxidative stress-induced factors from vascular smooth muscle cells. J Biol Chem. 2000;275(1):189-96.
Damsker JM, Bukrinsky MI, Constant SL. Preferential chemotaxis of activated human CD4+ T cells by extracellular cyclophilin A. J Leukoc Biol 2007; 82: 613-8. doi:10.1189/jlb.0506317.

Citation

Sahin A, Asik O, Tatli O, Karaca Y, Demir S, Mentese A, et al. The diagnostic value of cyclophilin A and VAP-1 in patients with suspected pulmonary embolism. Ortadogu Tıp Derg. 2018;10(3):228-33. https://doi.org/10.21601/ortadogutipdergisi.441377

Sahin, A., Asik, O., Tatli, O., Karaca, Y., Demir, S., Mentese, A., Karahan, S. C., & Turedi, S. (2018). The diagnostic value of cyclophilin A and VAP-1 in patients with suspected pulmonary embolism. Ortadoğu Tıp Dergisi, 10(3), 228-233. https://doi.org/10.21601/ortadogutipdergisi.441377

Sahin, A., Asik, O., Tatli, O., Karaca, Y., Demir, S., Mentese, A., . . . Turedi, S. (2018). The diagnostic value of cyclophilin A and VAP-1 in patients with suspected pulmonary embolism. Ortadoğu Tıp Dergisi, 10(3), pp. 228-233. https://doi.org/10.21601/ortadogutipdergisi.441377

Sahin A, Asik O, Tatli O, et al. The diagnostic value of cyclophilin A and VAP-1 in patients with suspected pulmonary embolism. Ortadogu Tıp Derg. 2018;10(3), 228-233. https://doi.org/10.21601/ortadogutipdergisi.441377

Sahin, Aynur, Olgun Asik, Ozgur Tatli, Yunus Karaca, Selim Demir, Ahmet Mentese, Suleyman Caner Karahan, and Suleyman Turedi. "The diagnostic value of cyclophilin A and VAP-1 in patients with suspected pulmonary embolism". Ortadoğu Tıp Dergisi 2018 10 no. 3 (2018): 228-233. https://doi.org/10.21601/ortadogutipdergisi.441377

Sahin, Aynur et al. "The diagnostic value of cyclophilin A and VAP-1 in patients with suspected pulmonary embolism". Ortadoğu Tıp Dergisi, vol. 10, no. 3, 2018, pp. 228-233. https://doi.org/10.21601/ortadogutipdergisi.441377